Assist. Prof. Samo Hudoklin, PhD
+386 1 543 7687
E-mail: samo.hudoklin@mf.uni-lj.si
Research interests
I work as an assistant professor, full-time employee at the Institute of Cell Biology, Faculty of Medicine, University of Ljubljana, Slovenia. I teach courses in cell biology and co-author dedicated textbooks for students. My research work focuses on the studies of membrane organization and its transport in the normal, pathological and regenerating urinary bladder epithelium, i.e. the urothelium. In particular, the role of the Golgi apparatus and post-Golgi compartments in the formation of urothelial plaques. My scientific work is done on in vitro and animal models using a wide range of light and electron microscopy techniques, with emphasis on cryo-techniques, 3D techniques and immunostaining. I help to promote the development of microscopy techniques and take care of the equipment. I collaborate with various global partners, participate in international conferences and am a board member of Slovenian Society for Microscopy.
My research intrests include microscopic analyses of urothelial cells' compartments (normal differentiation, during regeneration, in pathology, old), membrane trafficking, Golgi apparatus and post-Golgi compartments, mitochondria, endocytosis, bladder stretching, extracellular vesicles and nanoparticles.
Methodological expertise
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Light microscopy (including fluorescence)
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transmission- and scanning electron microscopy
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sample preparation for light- and electron microscopy (chemical fixation, cryo-techniques including freeze-substitution)
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cryo-sectioning (including Tokuyasu method)
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immuno(-electron) microscopy
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3D electron microscopy methods (tomography, FIB-SEM, array tomography)
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automatic segmentation of electron microscopy volumetric data
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freeze-fracturing
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correlative microscopy