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Prof. Rok Romih, PhD

+386 1 543 7683

E-mail: rok.romih@mf.uni-lj.si

Research interests (key words)

Cell membranes, membrane proteins, mechanosensors, vesicular traffic, endocytosis, exocytosis, autophagy, Golgi apparatus, endosomes, lysosome, cytoskeleton, nanoparticles, interstitial cystitis, urothelial carcinoma, sample preparation for electron microscopy, immuno-electron microscopy, 3D reconstructions in microscopy.

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Leader of the research programme P3-0108 Differentiation of urothelial cells (2009 do 2023).

Methodological expertise​

  • sample preparation for electron microscopy

  • cryofixation

  • freeze-substitution

  • low temperature embedding

  • freeze-fracturing

  • Tokuyasu technique

  • immuno-electron microscopy

  • immunofluorescence.

Education and Degrees

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1990      B.S. Biology (University of Ljubljana)

1993      M.S. Cell Biology (University of Ljubljana)

1996      Ph.D. Medicine (University of Ljubljana)

Positions

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1990-present       Faculty of Medicine, University of Ljubljana

1990-1996            Young Researcher

1994-2000            Teaching Assistant

2000-2009            Assistant Professor

2009-2015            Associate Professor

2015-present       Professor

Other activities

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  • President of the Slovene Society for Microscopy (2003-2011)

  • Member of the European Microscopy Society

  • Coordinator of research field Basic medicine at University Doctoral Programme in Biomedicine

Selected publications

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  • KURET, Tadeja, ERDANI-KREFT, Mateja, ROMIH, Rok, VERANIČ, Peter. Cannabidiol as a promising therapeutic option in IC/BPS : in vitro evaluation of its protective effects against inflammation and oxidative stress. International journal of molecular sciences. 2023, vol. 24, iss. 5, str. [1]-20, ilustr. ISSN 1422-0067. https://www.mdpi.com/1422-0067/24/5/5055, DOI: 10.3390/ijms24055055. [COBISS.SI-ID 144226307]

  • RESNIK, Nataša, ROMIH, Rok, ERDANI-KREFT, Mateja, HUDOKLIN, Samo. Freeze-fracture electron microscopy for extracellular vesicle analysis. Journal of visualized experiments. 2022, vol. 187, str. 1-13, ilustr. ISSN 1940-087X. https://www.jove.com/t/63550/freeze-fracture-electron-microscopy-for-extracellular-vesicle-analysis, DOI: 10.3791/63550. [COBISS.SI-ID 125862659]

  • ERMAN, Andreja, KAMENŠEK, Urška, DRAGIN JERMAN, Urška, PAVLIN, Mojca, ČEMAŽAR, Maja, VERANIČ, Peter, ROMIH, Rok. How cancer cells invade bladder epithelium and form tumors : the mouse bladder tumor model as a model of tumor recurrence in patients. International journal of molecular sciences. 2021, vol. 22, iss. 12, str. 1-20, ilustr. ISSN 1422-0067. https://www.mdpi.com/1422-0067/22/12/6328, DOI: 10.3390/ijms22126328. [COBISS.SI-ID 67627523]

  • ZUPANČIČ, Daša, KORAĆ-PRLIĆ, Jelena, ERDANI-KREFT, Mateja, FRANKOVIĆ, Lucija, VILOVIĆ, Katarina, JERUC, Jera, ROMIH, Rok, TERZIĆ, Janoš. Vitamin A rich diet diminishes early urothelial carcinogenesis by altering retinoic acid signalling. Cancers. 2020, vol. 12, iss. 7, str. 1-19, ilustr. ISSN 2072-6694. https://www.mdpi.com/2072-6694/12/7/1712, DOI: 10.3390/cancers12071712. [COBISS.SI-ID 21184515]

  • ŽEROVNIK MEKUČ, Manca, BOHAK, Ciril, HUDOKLIN, Samo, KIM, Byeong Hak, ROMIH, Rok, KIM, Min Young, MAROLT, Matija. Automatic segmentation of mitochondria and endolysosomes in volumetric electron microscopy data. Computers in Biology and Medicine. [Print ed.]. Apr. 2020, vol. 119, str. 1-11, ilustr. ISSN 0010-4825. https://www.sciencedirect.com/science/article/pii/S0010482520300792?dgcid=author, DOI: 10.1016/j.compbiomed.2020.103693. [COBISS.SI-ID 1538555331]

  • LIAO, Yi, THAM, Daniel K. L., LIANG, Feng-Xia, CHANG, Jennifer, WEI, Yuan, SUDHIR, Putty-Reddy, SALL, Joseph, REN, Sarah J., CHICOTE, Javier U., ROMIH, Rok, et al. Mitochondrial lipid droplet formation as a detoxification mechanism to sequester and degrade excessive urothelial membranes. Molecular biology of the cell. Nov. 2019, vol. 30, iss. 24, str. 2969-2984, ilustr. ISSN 1059-1524. https://www.molbiolcell.org/doi/pdf/10.1091/mbc.E19-05-0284, DOI: 10.1091/mbc.E19-05-0284. [COBISS.SI-ID 15006211]

  • MANČEK KEBER, Mateja, LAINŠČEK, Duško, BENČINA, Mojca, CHEN, Jiaji G., ROMIH, Rok, HUNTER, Zachary R., TREON, Steven P., JERALA, Roman. Extracellular vesicle-mediated transfer of constitutively active MyD88L265PMyD88L265P engages MyD88wtMyD88wt and activates signalling. Blood. 12. Apr. 2018, vol. 131, iss. 15, str. 1720-1729. ISSN 0006-4971. http://www.bloodjournal.org/content/early/2018/01/19/blood-2017-09-805499/tab-e-letters, DOI: 10.1182/blood-2017-09-805499. [COBISS.SI-ID 6319130]

  • WANKEL, Bret, OUYANG, Jiangyong, GUO, Xuemei, HADJIOLOVA, Krassimira, MILLER, Jeremy, LIAO, Yi, LONG THAM, Daniel Kai, ROMIH, Rok, ANDRADE, Leonardo R., GUMPER, Iwona, et al. Sequential and compartmentalized action of Rabs, SNAREs, and MAL in the apical delivery of fusiform vesicles in urothelial umbrella cells. Molecular biology of the cell. May 2016, vol. 27, iss. 10, str. 1621-1634, ilustr. ISSN 1059-1524. http://www.molbiolcell.org/content/27/10/1621.long, DOI: 10.1091/mbc.E15-04-0230. [COBISS.SI-ID 33075417]

  • VIEIRA, Neide, DENG, Fang-Ming, LIANG, Feng-Xia, LIAO, Yi, CHANG, Jennifer, ZHOU, Ge, ZHENG, Weiyue, SIMON, Jean-Pierre, DING, Mingxiao, WU, Xue-Ru, ROMIH, Rok, KREIBICH, Gert, SUN, Tung-Tien. SNX31 : a novel sorting nexin associated with the uroplakin-degrading multivesicular bodies in terminally differentiated urothelial cells. PloS one. Jun. 2014, vol. 9, iss. 6. ISSN 1932-6203. http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0099644, DOI: 10.1371/journal.pone.0099644. [COBISS.SI-ID 31861721]

  • PRIJIČ, Sara, PROSEN, Lara, ČEMAŽAR, Maja, ŠČANČAR, Janez, ROMIH, Rok, LAVRENČAK, Jaka, BREGAR, Vladimir Boštjan, CÖR, Andrej, KRŽAN, Mojca, ŽNIDARŠIČ, Andrej, SERŠA, Gregor. Surface modified magnetic nanoparticles for immuno-gene therapy of murine mammary adenocarcinoma. Biomaterials. [Print ed.]. 2012, vol. 33, iss. 17, str. 4379-4391. ISSN 0142-9612. DOI: 10.1016/j.biomaterials.2012.02.061. [COBISS.SI-ID 1237883]

  • ZHOU, Ge, LIANG, Feng-Xia, ROMIH, Rok, et al. MAL facilitates the incorporation of exocytic uroplakin-delivering vesicles into the apical membrane of urothelial umbrella cells. Molecular biology of the cell. 2012, vol. 23, iss. 7, str. 1354-1366, ilustr. ISSN 1059-1524. http://www.molbiolcell.org/content/23/7/1354.long. [COBISS.SI-ID 30079193]

  • HUDOKLIN, Samo, JEZERNIK, Kristijan, NEUMÜLLER, Josef, PAVELKA, Margit, ROMIH, Rok. Electron tomography of fusiform vesicles and their organization in urothelial cells. PloS one. 2012, vol. 7, iss. 3, str. [1-8], e32935. ISSN 1932-6203. http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0032935, DOI: 10.1371/journal.pone.0032935. [COBISS.SI-ID 30076889]

  • DERGANC, Jure, BOŽIČ, Bojan, ROMIH, Rok. Shapes of discoid intracellular compartments with small relative volumes. PloS one. 2011, vol. 6, issue 11, str. [1-9]. e26824. ISSN 1932-6203. http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0026824, DOI: 10.1371/journal.pone.0026824. [COBISS.SI-ID 29195737]

  • ERDANI-KREFT, Mateja, ROMIH, Rok, KREFT, Marko, JEZERNIK, Kristijan. Endocytotic activity of bladder superficial urothelial cells is inversely related to their differentiation stage. Differentiation. 2009, letn. 77, št. 1, str. 48-59. ISSN 0301-4681. DOI: 10.1016/j.diff.2008.09.011. [COBISS.SI-ID 25479641]

  • ROMIH, Rok, KOROŠEC, Peter, DE MELLO, Wilson, JEZERNIK, Kristijan. Differentiation of epithelial cells in the urinary tract. Cell and tissue research. 2005, letn. 320, str. 259-268. ISSN 0302-766X. [COBISS.SI-ID 19282649]

  • LIANG, Feng-Xia, BOSLAND, Maarten C., HUANG, Hongying, ROMIH, Rok, et al. Cellular basis of urothelial squamous metaplasia: roles of lineage heterogeneity and cell replacement. The Journal of cell biology. 2005, letn. 171, št. 5, str. 835-844. ISSN 0021-9525. [COBISS.SI-ID 20708569]

  • RUPNIK, Marjan, KREFT, Marko, SIKDAR, S... K., GRILC, Sonja, ROMIH, Rok, ZUPANČIČ, Gregor, MARTIN, TFJ, ZOREC, Robert. Rapid regulated dense-core vesicle exocytosis requires the CAPS protein. Proceedings of the National Academy of Sciences of the United States of America. May 2000, letn. 97, št. 10, str. 5627-5632. ISSN 0027-8424. [COBISS.SI-ID 11498201]

  • ROMIH, Rok, JEZERNIK, Kristijan, MAŠERA, Andrej. Uroplakins and cytokeratins in the regenerating rat urothelium after sodium saccharin treatment. Histochemistry and cell biology. 1998, let. 109, str. 263-269. ISSN 0948-6143. [COBISS.SI-ID 7556569]

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